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anti mouse igg2a isotype control  (Bio X Cell)


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    Bio X Cell anti mouse igg2a isotype control
    Anti Mouse Igg2a Isotype Control, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 98/100, based on 1784 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+igg2a+isotype+control/InVivoMAb+rat+IgG2a+isotype+control%2C+anti-trinitrophenol/pm41932811-45-11-20
    Average 98 stars, based on 1784 article reviews
    anti mouse igg2a isotype control - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Injection:

    Article Title: Th9-endothelial cell crosstalk promotes inflammatory atherosclerotic cardiovascular disease
    Article Snippet: .. In some cases, mice were injected with mouse IL-9 blocking antibody (BioXCell, BE0181) or mouse IgG2a isotype control (BioXCell, BE0085) at 100μg in 200ul two times a week. for the IMQ and IL-23 (induced) psoriasis models, IL-9-blocking antibodies were used starting on d1 on psoriasis induction and continued until mice were euthanized. ..

    Article Title: NK cells promote cardiac cell death and regulate myelopoiesis in myocardial infarction.
    Article Snippet: .. To evaluate the role of NK cells in post-ischemic cardiac remodeling, C57BL/6J Wild type mice were injected i.p. with a depleting mouse anti-mouse NK1.1 monoclonal antibody (200g/mouse, BioxCell BE0036 clone PK136) or with a mouse IgG2a isotype control (200g/mouse BioxCell BE0085 clone 2A3) 1 hour after coronary artery ligation. ..

    Blocking Assay:

    Article Title: Th9-endothelial cell crosstalk promotes inflammatory atherosclerotic cardiovascular disease
    Article Snippet: .. In some cases, mice were injected with mouse IL-9 blocking antibody (BioXCell, BE0181) or mouse IgG2a isotype control (BioXCell, BE0085) at 100μg in 200ul two times a week. for the IMQ and IL-23 (induced) psoriasis models, IL-9-blocking antibodies were used starting on d1 on psoriasis induction and continued until mice were euthanized. ..

    Control:

    Article Title: Th9-endothelial cell crosstalk promotes inflammatory atherosclerotic cardiovascular disease
    Article Snippet: .. In some cases, mice were injected with mouse IL-9 blocking antibody (BioXCell, BE0181) or mouse IgG2a isotype control (BioXCell, BE0085) at 100μg in 200ul two times a week. for the IMQ and IL-23 (induced) psoriasis models, IL-9-blocking antibodies were used starting on d1 on psoriasis induction and continued until mice were euthanized. ..

    Article Title: Preclinical characterisation of the protective capacity of an anti-nucleoprotein hRSV monoclonal antibody.
    Article Snippet: .. Mice immunisation with the murine anti-N-hRSV antibody and viral challenge Male 6–8-week-old BALB/c mice were intraperitoneally treated with 200 μg of murine anti-N-hRSV mAb (clone 1E9/D1), palivizumab (Synagis®; AstraZeneca), a mouse IgG2a isotype control (BioXCell; cat #BE0085; RRID: AB_1107771), or phosphate buffered saline (PBS) as the vehicle on day −1. ..

    Article Title: NK cells promote cardiac cell death and regulate myelopoiesis in myocardial infarction.
    Article Snippet: .. To evaluate the role of NK cells in post-ischemic cardiac remodeling, C57BL/6J Wild type mice were injected i.p. with a depleting mouse anti-mouse NK1.1 monoclonal antibody (200g/mouse, BioxCell BE0036 clone PK136) or with a mouse IgG2a isotype control (200g/mouse BioxCell BE0085 clone 2A3) 1 hour after coronary artery ligation. ..

    Article Title: Non-neutralizing antibodies to influenza A matrix-protein-2-ectodomain are broadly effective therapeutics and resistant to viral escape mutations
    Article Snippet: The 96-well plates were coated with the indicated antigens, such as M2e peptides (5 μg/ml; M2e vaccine or CS), or recombinant mouse Fc gamma RI, RIIB, RIII, or RIV (5 μg/ml; R&D Systems) in 15 mM Na 2 CO 3 and 35 mM NaHCO 3 bicarbonate buffer (pH 9.6) at 4°C overnight. .. The plates were then blocked with PBS + 1% BSA for 1 hour at room temperature before the specified M2e antibodies, mouse IgG1 isotype control (clone: MOPC-21, BioXCell), or mouse IgG2a isotype control (clone: C1.18.4, BioXCell) was added (all 50 μg/ml) in PBS-T (0.1% Tween 20) and incubated for 1.5 hours. .. The plates were washed three times with PBS-T, and goat anti-mouse IgG-HRP (Jackson ImmunoResearch, USA) was added and incubated for 1 hour.

    Article Title: A monoclonal antibody that inhibits the shedding of CD16a and CD16b and promotes antibody-dependent cellular cytotoxicity against tumors.
    Article Snippet: • Mouse IgG1 isotype control, BioXCell BE0083 (RRID:AB_1107784) and Biolegend 400197 (RRID:AB_2927801). .. • Mouse IgG2a isotype control, Bio XCell BE0085 (RRID:AB_1107771). .. • Mouse IgG2b isotype control, Biolegend 400369.

    Article Title: Preclinical characterisation of the protective capacity of an anti-nucleoprotein hRSV monoclonal antibody
    Article Snippet: .. Male 6–8-week-old BALB/c mice were intraperitoneally treated with 200 μg of murine anti-N-hRSV mAb (clone 1E9/D1), palivizumab (Synagis®; AstraZeneca), a mouse IgG2a isotype control (BioXCell; cat #BE0085; RRID: AB_1107771 ), or phosphate buffered saline (PBS) as the vehicle on day −1. ..

    Article Title: Anti-Peptidylarginine-4 Autoantibodies Derived from Patients with Rheumatoid Arthritis Exert Pathogenic Effects by Activating Monocytes and Exacerbating Inflammatory Arthritis.
    Article Snippet: Objective: Autoantibodies targeting peptidylarginine deiminase 4 (PAD4), an enzyme involved in protein citrullination, are found in a subset of rheumatoid arthritis (RA) patients with severe joint disease.. However, the mechanisms by which anti-PAD4 antibodies participate in disease pathogenesis are incompletely defined.. Methods: We investigated the role of anti-PAD4 monoclonal antibodies derived from RA patients using a collagen-induced arthritis (CIA) mouse model and human monocyte in vitro cultures.

    other:

    Article Title: Non-neutralizing antibodies to influenza A matrix-protein-2-ectodomain are broadly effective therapeutics and resistant to viral escape mutations.
    Article Snippet: After 1 hour, the plates were washed three times with PBS- T and added with mouse (0.5 μg/ml) C1q protein (Complement Technology) in PBS- T. After a 1- hour incubation at room temperature, the plates were washed three times with PBS- T, added with biotinylated antimouse C1q antibody (Thermo Fisher Scientific), and incubated for 1 hour.

    Mouse Assay:

    Article Title: Preclinical characterisation of the protective capacity of an anti-nucleoprotein hRSV monoclonal antibody.
    Article Snippet: .. Mice immunisation with the murine anti-N-hRSV antibody and viral challenge Male 6–8-week-old BALB/c mice were intraperitoneally treated with 200 μg of murine anti-N-hRSV mAb (clone 1E9/D1), palivizumab (Synagis®; AstraZeneca), a mouse IgG2a isotype control (BioXCell; cat #BE0085; RRID: AB_1107771), or phosphate buffered saline (PBS) as the vehicle on day −1. ..

    Saline:

    Article Title: Preclinical characterisation of the protective capacity of an anti-nucleoprotein hRSV monoclonal antibody.
    Article Snippet: .. Mice immunisation with the murine anti-N-hRSV antibody and viral challenge Male 6–8-week-old BALB/c mice were intraperitoneally treated with 200 μg of murine anti-N-hRSV mAb (clone 1E9/D1), palivizumab (Synagis®; AstraZeneca), a mouse IgG2a isotype control (BioXCell; cat #BE0085; RRID: AB_1107771), or phosphate buffered saline (PBS) as the vehicle on day −1. ..

    Article Title: Preclinical characterisation of the protective capacity of an anti-nucleoprotein hRSV monoclonal antibody
    Article Snippet: .. Male 6–8-week-old BALB/c mice were intraperitoneally treated with 200 μg of murine anti-N-hRSV mAb (clone 1E9/D1), palivizumab (Synagis®; AstraZeneca), a mouse IgG2a isotype control (BioXCell; cat #BE0085; RRID: AB_1107771 ), or phosphate buffered saline (PBS) as the vehicle on day −1. ..

    Ligation:

    Article Title: NK cells promote cardiac cell death and regulate myelopoiesis in myocardial infarction.
    Article Snippet: .. To evaluate the role of NK cells in post-ischemic cardiac remodeling, C57BL/6J Wild type mice were injected i.p. with a depleting mouse anti-mouse NK1.1 monoclonal antibody (200g/mouse, BioxCell BE0036 clone PK136) or with a mouse IgG2a isotype control (200g/mouse BioxCell BE0085 clone 2A3) 1 hour after coronary artery ligation. ..

    Incubation:

    Article Title: Non-neutralizing antibodies to influenza A matrix-protein-2-ectodomain are broadly effective therapeutics and resistant to viral escape mutations
    Article Snippet: The 96-well plates were coated with the indicated antigens, such as M2e peptides (5 μg/ml; M2e vaccine or CS), or recombinant mouse Fc gamma RI, RIIB, RIII, or RIV (5 μg/ml; R&D Systems) in 15 mM Na 2 CO 3 and 35 mM NaHCO 3 bicarbonate buffer (pH 9.6) at 4°C overnight. .. The plates were then blocked with PBS + 1% BSA for 1 hour at room temperature before the specified M2e antibodies, mouse IgG1 isotype control (clone: MOPC-21, BioXCell), or mouse IgG2a isotype control (clone: C1.18.4, BioXCell) was added (all 50 μg/ml) in PBS-T (0.1% Tween 20) and incubated for 1.5 hours. .. The plates were washed three times with PBS-T, and goat anti-mouse IgG-HRP (Jackson ImmunoResearch, USA) was added and incubated for 1 hour.

    Concentration Assay:

    Article Title: Anti-Peptidylarginine-4 Autoantibodies Derived from Patients with Rheumatoid Arthritis Exert Pathogenic Effects by Activating Monocytes and Exacerbating Inflammatory Arthritis.
    Article Snippet: Objective: Autoantibodies targeting peptidylarginine deiminase 4 (PAD4), an enzyme involved in protein citrullination, are found in a subset of rheumatoid arthritis (RA) patients with severe joint disease.. However, the mechanisms by which anti-PAD4 antibodies participate in disease pathogenesis are incompletely defined.. Methods: We investigated the role of anti-PAD4 monoclonal antibodies derived from RA patients using a collagen-induced arthritis (CIA) mouse model and human monocyte in vitro cultures.



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    (A) Experimental design of the anti-IL-1β antibody study in the FOP mouse model. Mice received weekly intraperitoneal injections beginning at P27 (day 0) of either vehicle (saline), <t>IgG2A</t> isotype control antibody, or the anti-IL-1β monoclonal antibody 01BSUR (10 mg/kg). Trauma-induced HO was generated using a ball-drop injury model applied to the right hind limb at P28. HO formation was quantified by µCT at day 21. Created with Biorender.com. (B) Quantification of trauma-induced HO volume measured by µCT. Each point represents an individual mouse, and boxes show group distributions. Treatment with the anti-IL-1β antibody 01BSUR significantly reduced HO volume compared with both vehicle-treated mice (p = 0.0087) and IgG2A isotype controls (p = 0.0095). (C) Representative three-dimensional µCT reconstructions of skeletal structures from each treatment group. Areas of HO formation are highlighted. Extensive HO is observed in vehicle- and IgG2A-treated mice, whereas mice treated with the anti-IL-1β antibody show markedly reduced HO at the injury site. Representative images were chosen based on the mean HO volume of each group.
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    Image Search Results


    (A) Experimental design of the anti-IL-1β antibody study in the FOP mouse model. Mice received weekly intraperitoneal injections beginning at P27 (day 0) of either vehicle (saline), IgG2A isotype control antibody, or the anti-IL-1β monoclonal antibody 01BSUR (10 mg/kg). Trauma-induced HO was generated using a ball-drop injury model applied to the right hind limb at P28. HO formation was quantified by µCT at day 21. Created with Biorender.com. (B) Quantification of trauma-induced HO volume measured by µCT. Each point represents an individual mouse, and boxes show group distributions. Treatment with the anti-IL-1β antibody 01BSUR significantly reduced HO volume compared with both vehicle-treated mice (p = 0.0087) and IgG2A isotype controls (p = 0.0095). (C) Representative three-dimensional µCT reconstructions of skeletal structures from each treatment group. Areas of HO formation are highlighted. Extensive HO is observed in vehicle- and IgG2A-treated mice, whereas mice treated with the anti-IL-1β antibody show markedly reduced HO at the injury site. Representative images were chosen based on the mean HO volume of each group.

    Journal: bioRxiv

    Article Title: Gut microbiome-dependent IL-1 signaling is a mediator of ACVR1 R206H -driven heterotopic ossification

    doi: 10.64898/2026.04.05.716562

    Figure Lengend Snippet: (A) Experimental design of the anti-IL-1β antibody study in the FOP mouse model. Mice received weekly intraperitoneal injections beginning at P27 (day 0) of either vehicle (saline), IgG2A isotype control antibody, or the anti-IL-1β monoclonal antibody 01BSUR (10 mg/kg). Trauma-induced HO was generated using a ball-drop injury model applied to the right hind limb at P28. HO formation was quantified by µCT at day 21. Created with Biorender.com. (B) Quantification of trauma-induced HO volume measured by µCT. Each point represents an individual mouse, and boxes show group distributions. Treatment with the anti-IL-1β antibody 01BSUR significantly reduced HO volume compared with both vehicle-treated mice (p = 0.0087) and IgG2A isotype controls (p = 0.0095). (C) Representative three-dimensional µCT reconstructions of skeletal structures from each treatment group. Areas of HO formation are highlighted. Extensive HO is observed in vehicle- and IgG2A-treated mice, whereas mice treated with the anti-IL-1β antibody show markedly reduced HO at the injury site. Representative images were chosen based on the mean HO volume of each group.

    Article Snippet: IgG2A isotype antibody from R&D Systems (catalog no. MAB0031) was used as a control.

    Techniques: Saline, Control, Generated